Sample(30 ug whole cell lysate)
A:HeLa S3(GTX14654)
5% SDS PAGE
GTX107921 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using ASK1(GTX107921) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

ASK1 antibody [C2C3-2], C-term detects MAP3K5 protein by Western blot analysis.
A. 50 μg mouse lung lysate/extract
5 % SDS-PAGE
ASK1 antibody [C2C3-2], C-term (GTX107921) dilution: 1:1000

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ASK1 antibody [C2C3-2], C-term (GTX107921) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the journal Sci Rep in 2017.PMID: 28526890

The data was published in the journal PLoS One in 2012.PMID: 22470469