Untreated (–) and treated (+) 293T whole cell extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATF1 (phospho Ser63) antibody (GTX130420) diluted at 1:5000.

Untreated (–) and treated (+) A431 whole cell extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATF1 (phospho Ser63) antibody (GTX130420) diluted at 1:5000.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ATF1 (phospho Ser63) antibody (GTX130420) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.