ICC/IF analysis of HeLa cells using GTX00687 ATF6 antibody [1-7].
Green : Primary antibody
Blue : DAPI
Dilution : 1:100

WB analysis of various samples using GTX00687 ATF6 antibody [1-7]. The asterisk denotes an unglycosylated form of pATF6α(P). ATF6α is constitutively expressed as pATF6α(P), and converted to pATF6α(N) ( > 50-kDa protein) in ER-stressed cells.
pATF6α(P) : the endogenous precursor ATF6 alpha (~90kDa)
pATF6α(N) : the cleaved product of ATF6 alpha (>50kDa)
Tm: cells treated with 2 μg/ml tunicamycin (inhibitor of N-glycosylation)< br>DTT: cells treated with 1mM dithiothreitol (reducing reagent).
Loading : 50μg

IP analysis of HeLa or 293 cell lysates using GTX00687 ATF6 antibody [1-7]. It is recommended using a light chain specific anti-mouse IgG antibody as a secondary antibody.
Lane 1, 2, 7, and 8 : Input
Lanes 3, 4, 9, and 10 : Non-related IgG as IP antibody
Lanes 5, 6, 11, and 12 : GTX00687 as IP antibody
IB antibody: GTX00687 ATF6 antibody [1-7]