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ATF6 antibody [1-7]

Orthogonal
Anti-ATF6 antibody [1-7] used in Immunoprecipitation (IP). GTX00687
Anti-ATF6 antibody [1-7] used in Western Blot (WB). GTX00687
Anti-ATF6 antibody [1-7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00687

Cat. No. GTX00687

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1-7

Isotype

 IgG2a

Application

 WB, ICC/IF, IP

Reactivity

 Human
Package
50 μg ($399)
See all ATF6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:500
IP 1:100-1:500
Not tested in other applications.

Calculated MW

75 kDa. ( Note )

Product Note

This antibody detects both full-length ATF6 alpha as well as the cleaved ATF6 alpha p50 form.

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human ATF6 alpha; (His-tagged amino-terminal fragment of ATF6 alpha)

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

activating transcription factor 6 , ACHM7 , ATF6A

Background

This gene encodes a transcription factor that activates target genes for the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Although it is a transcription factor, this protein is unusual in that it is synthesized as a transmembrane protein that is embedded in the ER. It functions as an ER stress sensor/transducer, and following ER stress-induced proteolysis, it functions as a nuclear transcription factor via a cis-acting ER stress response element (ERSE) that is present in the promoters of genes encoding ER chaperones. This protein has been identified as a survival factor for quiescent but not proliferative squamous carcinoma cells. There have been conflicting reports about the association of polymorphisms in this gene with diabetes in different populations, but another polymorphism has been associated with increased plasma cholesterol levels. This gene is also thought to be a potential therapeutic target for cystic fibrosis. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-ATF6 antibody [1-7] used in Immunoprecipitation (IP). GTX00687

GTX00687 IP Image

Orthogonal

IP analysis of HeLa or 293 cell lysates using GTX00687 ATF6 antibody [1-7]. It is recommended using a light chain specific anti-mouse IgG antibody as a secondary antibody.
Lane 1, 2, 7, and 8 : Input
Lanes 3, 4, 9, and 10 : Non-related IgG as IP antibody
Lanes 5, 6, 11, and 12 : GTX00687 as IP antibody
IB antibody: GTX00687 ATF6 antibody [1-7]

Anti-ATF6 antibody [1-7] used in Western Blot (WB). GTX00687

GTX00687 WB Image

Orthogonal

WB analysis of various samples using GTX00687 ATF6 antibody [1-7]. The asterisk denotes an unglycosylated form of pATF6α(P). ATF6α is constitutively expressed as pATF6α(P), and converted to pATF6α(N) ( > 50-kDa protein) in ER-stressed cells.
pATF6α(P) : the endogenous precursor ATF6 alpha (~90kDa)
pATF6α(N) : the cleaved product of ATF6 alpha (>50kDa)
Tm: cells treated with 2 μg/ml tunicamycin (inhibitor of N-glycosylation)< br>DTT: cells treated with 1mM dithiothreitol (reducing reagent).
Loading : 50μg

Anti-ATF6 antibody [1-7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00687

GTX00687 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX00687 ATF6 antibody [1-7].
Green : Primary antibody
Blue : DAPI
Dilution : 1:100

REFERENCE

Clone Reference

Mori K et al. Mol Biol Cell. 2010; 21 (9):1435-8 Divest yourself of a preconceived idea: transcription factor ATF6 is not a soluble protein!
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399