WB analysis of NIH-3T3 cell lysate using GTX00686 ATF6 antibody [37-1]. The asterisk denotes an unglycosylated form of pATF6α(P). ATF6α is constitutively expressed as pATF6α(P), and converted to pATF6α(N) ( > 50-kDa protein) in ER-stressed cells.
pATF6α(P) : the endogenous precursor ATF6α (~90kDa)
pATF6α(N) : the cleaved product of ATF6α(>50kDa)
Lane 1 : untreated
Lane 2 : 1mM DTT (reducing reagent) for 1 h
Lane 3 : 2 μg/ml tunicamycin (inhibitor of N-glycosylation) for 3 h
Lane 4 : 2 μg/ml tunicamycin (inhibitor of N-glycosylation) for 7 h.
Loading : 50μg

WB analysis of HeLa cell lysate using GTX00686 ATF6 antibody [37-1]. The asterisk denotes an unglycosylated form of pATF6α(P). ATF6α is constitutively expressed as pATF6α(P), and converted to pATF6α(N) ( > 50-kDa protein) in ER-stressed cells.
pATF6α(P) : the endogenous precursor ATF6α (~90kDa)
pATF6α(N) : the cleaved product of ATF6α(>50kDa)
Lane 1 : untreated
Lane 2 : 1mM DTT (reducing reagent) for 1 h
Lane 3 : 2 μg/ml tunicamycin (inhibitor of N-glycosylation) for 3 h
Lane 4 : 2 μg/ml tunicamycin (inhibitor of N-glycosylation) for 7 h.
Loading : 50μg