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ATF6 antibody

KO/KD
Anti-ATF6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15457
Anti-ATF6 antibody used in IHC (Paraffin sections) (IHC-P). GTX15457
Anti-ATF6 antibody used in IHC (Paraffin sections) (IHC-P). GTX15457
Anti-ATF6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15457
Anti-ATF6 antibody used in Western Blot (WB). GTX15457
Anti-ATF6 antibody used in Western Blot (WB). GTX15457
Anti-ATF6 antibody used in Western Blot (WB). GTX15457
Anti-ATF6 antibody used in Western Blot (WB). GTX15457

Cat. No. GTX15457

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
50 μg ($399)
See all ATF6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 1 μg/mL
ICC/IF 10 μg/mL
IHC-P Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

75 kDa. ( Note )

Positive Control

A-20 Cell Slide(GTX25541)

Product Note

The immunogen sequence of this antibody is within the cleaved form of ATF6 alpha.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

ATF6 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human ATF6.The immunogen is located within amino acids 30 - 80 of ATF6.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

activating transcription factor 6 , ACHM7 , ATF6A

Background

ATF6 is an endoplasmic reticulum (ER) stress-regulated transmembrane transcription factor that activates the transcription of ER molecules.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-ATF6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15457

GTX15457 ICC/IF Image

ICC/IF analysis of PFA-fixed A549 cells using GTX15457 AFT6 antibody.
Green : Primary antibody
Blue : DAPI
Dilution : 20μg/ml

Anti-ATF6 antibody used in IHC (Paraffin sections) (IHC-P). GTX15457

GTX15457 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX15457 AFT6 antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 5μg/ml

Anti-ATF6 antibody used in IHC (Paraffin sections) (IHC-P). GTX15457

GTX15457 IHC-P Image

IHC-P analysis of rat brain tissue using GTX15457 AFT6 antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 5μg/ml

Anti-ATF6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15457

GTX15457 ICC/IF Image

ICC/IF analysis of PFA-fixed human testis cells using GTX15457 AFT6 antibody.
Green : Primary antibody
Blue : DAPI
Dilution : 20μg/ml

Anti-ATF6 antibody used in Western Blot (WB). GTX15457

GTX15457 WB Image

WB analysis of various whole cell lysates using GTX15457 AFT6 antibody.
Dilution : 1μg/ml
Loading : 15μg per lane

Anti-ATF6 antibody used in Western Blot (WB). GTX15457

GTX15457 WB Image

KO/KD

WB analysis of extracts from normal (control) and ATF6 knockout (KO) HeLa cell lysates using GTX15457 AFT6 antibody.
Dilution : 0.5μg/ml
Loading : 15μg per lane

Anti-ATF6 antibody used in Western Blot (WB). GTX15457

GTX15457 WB Image

WB analysis of mouse heart and mouse skeleton muscle tissue lysates using GTX15457 AFT6 antibody.
Dilution : 1μg/ml
Loading : 15μg per lane

Anti-ATF6 antibody used in Western Blot (WB). GTX15457

GTX15457 WB Image

WB analysis of rat kidney tissue lysates using GTX15457 AFT6 antibody.
Dilution : 1μg/ml
Loading : 15μg per lane

REFERENCE

Application Reference

Ge L et al. Front Immunol 2022; 13 : 965708 ATF6α contributes to rheumatoid arthritis by inducing inflammatory cytokine production and apoptosis resistance.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399