Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ATG12 antibody (GTX124181) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (50 μg of whole cell lysate)
A: Rat brain
12% SDS PAGE
GTX124181 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ATG12 antibody detects ATG12 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
ATG12 stained by ATG12 antibody (GTX124181) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

ATG12 antibody detects ATG12 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
ATG12 antibody (GTX124181) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunoprecipitation of Atg 12-Atg5 protein from 293T whole cell extracts using 5 μg of ATG12 antibody (GTX124181).
Western blot analysis was performed using ATG12 antibody (GTX124181) diluted at 1:1000.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Sample (30 μg of whole cell lysate)
A: NIH-3T3
12% SDS PAGE
GTX124181 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG12 antibody (GTX124181) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the journal Sci Rep in 2017.PMID: 28195146

The data was published in the journal Sci Rep in 2017. PMID: 28134285

The data was published in the journal Biomolecules in 2019.PMID: 31505885

The data was published in the journal Sci Rep in 2017.PMID: 28195146

The data was published in the journal Sci Rep in 2017.PMID: 28195146