Wild-type (WT) and ATG14 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG14 antibody (GTX119950) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

KIAA0831 antibody detects KIAA0831 protein by Western blot analysis.
A. 20 μg Huh7 whole cell lysate/extract
0 % SDS-PAGE
KIAA0831 antibody (GTX119950) dilution: 1:1500