Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG4C antibody (GTX129488) diluted at 1:1000.

Immunoprecipitation of ATG4C protein from 293T whole cell extracts using 5 μg of ATG4C antibody (GTX129488).
Western blot analysis was performed using ATG4C antibody (GTX129488).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

ATG4C antibody detects ATG4C protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
10 % SDS-PAGE
ATG4C antibody (GTX129488) dilution: 1:1000