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ATG5 antibody

Orthogonal
Anti-ATG5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113309
Anti-ATG5 antibody used in Western Blot (WB). GTX113309
Anti-ATG5 antibody used in IHC (Paraffin sections) (IHC-P). GTX113309
Anti-ATG5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113309
Anti-ATG5 antibody used in Western Blot (WB). GTX113309
Anti-ATG5 antibody used in Western Blot (WB). GTX113309
Anti-ATG5 antibody used in Western Blot (WB). GTX113309
Anti-ATG5 antibody used in Western Blot (WB). GTX113309
Anti-ATG5 antibody used in Western Blot (WB). GTX113309

Cat. No. GTX113309

Reference 15
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, PLA

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ATG5 products
PRODUCT

Summary

ATG5 antibody detects autophagy related 5 (ATG5), a ~32kDa key protein that contributes to the formation of autophagic vesicles when conjugated to ATG12 through the actions of ATG7 and ATG10. It plays key roles in mitochondrial function, lymphocyte biology, and axonal maintenance, among others. Dysregulation of ATG5 is associated with several diseases, including sporadic Parkinson's disease, childhood asthma, and cancers.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
PLA Assay dependent
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

NT2D1 , PC-3 , HeLa (chloroquine treatment for 24hr)

Predict Reactivity

Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ATG5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10633458

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

autophagy related 5 , APG5 , APG5-LIKE , APG5L , ASP , SCAR25 , hAPG5

Cellular Localization

Cytoplasm

Database

Research Area

DATA IMAGES

Anti-ATG5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113309

GTX113309 ICC/IF Image

ATG5 antibody detects ATG5 protein at autophagosome by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATG5 protein stained by ATG5 antibody (GTX113309) diluted at 1:1000.
Red: Phalloidin, a F-actin marker.
Anti-ATG5 antibody used in Western Blot (WB). GTX113309

GTX113309 WB Image

Orthogonal

ATG5 antibody detects ATG5 protein by western blot analysis. Un-treated (-) and treated (+, 50 μM chloroquine treatment for 24 hrs) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ATG5 antibody (GTX113309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATG5 antibody used in IHC (Paraffin sections) (IHC-P). GTX113309

GTX113309 IHC-P Image

ATG5 antibody detects ATG5 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
ATG5 antibody (GTX113309) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ATG5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113309

GTX113309 ICC/IF Image

ATG5 antibody detects ATG5 protein at autophagosome by immunofluorescent analysis.
Sample: HepG2 cells treated with 3μM thapsigargin 16 hrs (rigtht) and mock (left) and were fixed in ice-cold MeOH for 10 min, permeabilize with cooled acetone for 2 min for min.
Green: ATG5 protein stained by ATG5 antibody (GTX113309) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-ATG5 antibody used in Western Blot (WB). GTX113309

GTX113309 WB Image

Sample (30 μg of whole cell lysate)
A: NT2D1
B: PC-3
12% SDS PAGE
GTX113309 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATG5 antibody used in Western Blot (WB). GTX113309

GTX113309 WB Image

Orthogonal

The data was published in the 2022 in Cancer Cell Int. PMID: 35292022

Anti-ATG5 antibody used in Western Blot (WB). GTX113309

GTX113309 WB Image

Orthogonal

The data was published in the 2022 in Cancer Cell Int. PMID: 35292022

Anti-ATG5 antibody used in Western Blot (WB). GTX113309

GTX113309 WB Image

The data was published in the 2022 in Cancer Cell Int. PMID: 35292022

Anti-ATG5 antibody used in Western Blot (WB). GTX113309

GTX113309 WB Image

The data was published in the 2022 in Cancer Cell Int. PMID: 35292022

REFERENCE

Application Reference

Li J et al. iScience 2023; 26 (1) : 105858 Oxygen-carrying sequential preservation mitigates liver grafts ischemia-reperfusion injury.
Tiezhi Li et al. Cancer Cell Int 2022; 22 (1) : 120
Application : WB
Reactivity : Human
Tianjiao Zeng et al. Oncol Rep 2021; 46 (1)
Application : WB
Reactivity : Human
Wen-Jui Lee et al. Molecules 2021; 26 (21) Type-3 Hyaluronan Synthase Attenuates Tumor Cells Invasion in Human Mammary Parenchymal Tissues.
Application : WB
Reactivity : Human
Yang CC et al. Antioxidants (Basel) 2021; 10 (6) Green Tea Polyphenol Catechins Inhibit Coronavirus Replication and Potentiate the Adaptive Immunity and Autophagy-Dependent Protective Mechanism to Improve Acute Lung Injury in Mice.
Application : WB
Reactivity : Mouse
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Submit a Reference

REVIEW

ATG5 antibody Cat. No. GTX113309

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Anti-ATG5 antibody used in Western Blot (WB). GTX113309
Date : Anonymous submitted on 18-Dec-2023
Score :
Application Tested : WB
Sample Species : Hu
Sample : HT29 and HCT116 cells
Amount used : 10μg
Blocking : 5% non-fat milk in TBST, 4°C, 1Hr
Primary Antibody : 1:1000, 4°C, 15Hr
Anti-ATG5 antibody used in Western Blot (WB). GTX113309
Date : Anonymous submitted on 06-May-2021
Score :
Application Tested : WB
Sample Species : Ms
Sample : MEF cells
Amount used : 10 μg
Blocking : 5% skim milk in TBST, 27°C, 1Hr
Primary Antibody : 1:5000, 4°C, 12Hr
Secondary Antibody : GTX213110-01, 1:5000, 27°C, 2Hr
Lane Description : Two groups (DMEM and CM) of whole cell extracts (10 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ATG5 antibody (GTX113309) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Submit a Review
Application Reference
Li J et al. iScience 2023; 26 (1):105858 Oxygen-carrying sequential preservation mitigates liver grafts ischemia-reperfusion injury.
Tiezhi Li et al. Cancer Cell Int 2022; 22 (1):120
Application : WB
Reactivity : Human
Tianjiao Zeng et al. Oncol Rep 2021; 46 (1)
Application : WB
Reactivity : Human
Wen-Jui Lee et al. Molecules 2021; 26 (21) Type-3 Hyaluronan Synthase Attenuates Tumor Cells Invasion in Human Mammary Parenchymal Tissues.
Application : WB
Reactivity : Human
Yang CC et al. Antioxidants (Basel) 2021; 10 (6) Green Tea Polyphenol Catechins Inhibit Coronavirus Replication and Potentiate the Adaptive Immunity and Autophagy-Dependent Protective Mechanism to Improve Acute Lung Injury in Mice.
Application : WB
Reactivity : Mouse
Ha? A et al. Cells 2021; 10 (4) S6K1 Is Indispensible for Stress-Induced Microtubule Acetylation and Autophagic Flux.
Application : WB
Reactivity : Mouse
Nowosad A et al. Cell Death Dis 2021; 12 (5):481 p27 controls autophagic vesicle trafficking in glucose-deprived cells via the regulation of ATAT1-mediated microtubule acetylation.
Lin TY et al. Autophagy 2019;:1-18 BIRC5/Survivin is a novel ATG12-ATG5 conjugate interactor and an autophagy-induced DNA damage suppressor in human cancer and mouse embryonic fibroblast cells.
Application : PLA
Reactivity : Human
Wong CW et al. J Med Chem 2019; 62 (15):7111-7125 Toward Understanding the Antitumor Effects of Water-Soluble Fullerene Derivatives on Lung Cancer Cells: Apoptosis or Autophagy Pathways?
Application : WB
Reactivity : Human
Li FY et al. Glycobiology 2018; (Epub) Helicobacter pylori induces intracellular galectin-8 aggregation around damaged lysosomes within gastric epithelial cells in a host O-glycan-dependent manner.
Application : WB
Reactivity : Human
Chang HP et al. Int J Oncol 2018; (Epub) pterostilbene modulates the suppression of multidrug resistance protein-1 and triggers autophagic and apoptotic mechanisms in *-resistant human oral cancer car cells via akt signaling.
Application : WB
Reactivity : Human
Koh EH et al. J Biol Chem 2016; 291 (47):24747-24755 Mitochondrial Activity in Human White Adipocytes Is Regulated by the Ubiquitin Carrier Protein 9/microRNA-30a Axis.
Application : WB
Reactivity : Human
Hsieh YY et al. Am J Cancer Res 2016; 6 (8):1661-80 EZH2 inhibitors transcriptionally upregulate cytotoxic autophagy and cytoprotective unfolded protein response in human colorectal cancer cells.
Application : WB
Reactivity : Human
Lo MC et al. Am J Physiol Endocrinol Metab 2015; 309 (10):E829-39 nε-(carboxymethyl) lysine-induced mitochondrial fission and mitophagy cause decreased * secretion from β-cells.
Application : WB
Reactivity : Mouse
Lee KH et al. Sci Rep 2014; 4:6394 A gene expression signature-based approach reveals the mechanisms of action of the Chinese herbal medicine berberine.
Application : WB
Reactivity : Human
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169