Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1-RB] (GTX635395) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1-RB] (GTX635395) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1-RB] (GTX635395) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ATM antibody [2C1-RB] detects ATM protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
ATM stained by ATM antibody [2C1-RB] (GTX635395) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min