WB analysis of gamma-irradiated HeLa cells using GTX15666 ATM (phospho Ser1981) antibody [10H11].

ICC/IF analysis of HeLa cells left untreated (left panels) or treated with 10uM Camptothecin for 4 hours (right panels) using GTX15666 ATM (phospho Ser1981) antibody [10H11].
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes

ICC/IF analysis of HeLa cells irradiated with UV for 4 hours using GTX15666 ATM (phospho Ser1981) antibody [10H11]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Trito X-100 for 10 minutes

ICC/IF analysis of HT-29 cells using GTX15666 ATM (phospho Ser1981) antibody [10H11].
Left : Primary antibody
Right : Negative control without primary antibody
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
Dilution : 1:50 and incubated overnight

WB analysis of 40 ug of lysate from HeLa cells untreated (Lane 1), treated with 10uM Camptothecin for 4h (Lane 2) or 12h (Lane 3) using GTX15666 ATM (phospho Ser1981) antibody [10H11].
Dilution : 1:1000

ICC/IF analysis of 293 untreated cells (left panel) or stimulated cells with 100 mJ UV (right panel) using GTX15666 ATM (phospho Ser1981) antibody [10H11].
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes