Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM (phospho Ser1981) antibody (GTX132146) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ATM (phospho Ser1981) antibody detects ATM (phospho Ser1981) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
ATM (phospho Ser1981) stained by ATM (phospho Ser1981) antibody (GTX132146) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

ATM (phospho Ser1981) antibody detects ATM (phospho Ser1981) protein at nucleus by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATM (phospho Ser1981) stained by ATM (phospho Ser1981) antibody (GTX132146) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

ATM (phospho Ser1981) antibody detects ATM (phospho Ser1981) protein at nucleus by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATM (phospho Ser1981) stained by ATM (phospho Ser1981) antibody (GTX132146) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Scale bar= 10 μm.

Untreated (–) and treated (+) 293T whole cell extracts (60 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM (phospho Ser1981) antibody (GTX132146) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM (phospho Ser1981) antibody (GTX132146) diluted at 1:1000.

ATM (phospho Ser1981) antibody detects ATM (phospho Ser1981) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
ATM (phospho Ser1981) stained by ATM (phospho Ser1981) antibody (GTX132146) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

The data was published in the 2019 in J Exp Clin Cancer Res. PMID: 30961670