Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATP citrate lyase antibody [N1N2], N-term (GTX112387) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATP citrate lyase antibody [N1N2], N-term (GTX112387) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ATP citrate lyase antibody [N1N2], N-term detects ATP citrate lyase protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ATP citrate lyase protein stained by ATP citrate lyase antibody [N1N2], N-term (GTX112387) diluted at 1:500.
Blue: Hoechst 33342 staining.

ATP citrate lyase antibody [N1N2], N-term detects ATP citrate lyase protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
ATP citrate lyase stained by ATP citrate lyase antibody [N1N2], N-term (GTX112387) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemical analysis of paraffin-embedded Hepatoma, using ATP citrate lyase(GTX112387) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min