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ATP5J antibody [HL2934]

Recombinant antibody
Anti-ATP5J antibody [HL2934] used in Western Blot (WB). GTX640318
Anti-ATP5J antibody [HL2934] used in IHC (Paraffin sections) (IHC-P). GTX640318
Anti-ATP5J antibody [HL2934] used in Western Blot (WB). GTX640318
Anti-ATP5J antibody [HL2934] used in IHC (Paraffin sections) (IHC-P). GTX640318

Cat. No. GTX640318

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2934

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ATP5J products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human ATP5J recombinant protein.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATP synthase peripheral stalk subunit F6 , ATP5 , ATP5A , ATP5J , ATPM , CF6 , F6

Cellular Localization

Mitochondrion,Mitochondrion inner membrane

Background

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo complex has nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the F6 subunit of the Fo complex. The F6 subunit is required for F1 and Fo interactions. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. This gene has 1 or more pseudogenes. [provided by RefSeq, Feb 2016]

Database

Research Area

DATA IMAGES

Anti-ATP5J antibody [HL2934] used in Western Blot (WB). GTX640318

GTX640318 WB Image

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ATP5J antibody [HL2934] (GTX640318) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATP5J antibody [HL2934] used in IHC (Paraffin sections) (IHC-P). GTX640318

GTX640318 IHC-P Image

ATP5J antibody [HL2934] detects ATP5J protein by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney.
ATP5J stained by ATP5J antibody [HL2934] (GTX640318) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ATP5J antibody [HL2934] used in Western Blot (WB). GTX640318

GTX640318 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ATP5J antibody [HL2934] (GTX640318) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATP5J antibody [HL2934] used in IHC (Paraffin sections) (IHC-P). GTX640318

GTX640318 IHC-P Image

ATP5J antibody [HL2934] detects ATP5J protein by immunohistochemical analysis.
Sample: Paraffin-embedded human glioblastoma.
ATP5J stained by ATP5J antibody [HL2934] (GTX640318) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations