Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATP6V1A antibody (GTX110815) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ATP6V1A antibody detects ATP6V1A protein at cytoplasm on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
ATP6V1A antibody (GTX110815) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

ATP6V1A antibody detects ATP6V1A protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATP6V1A antibody (GTX110815) diluted by 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (20 μg of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX110815 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunoprecipitation of ATP6V1A protein from HeLa whole cell extracts using 5 μg of ATP6V1A antibody (GTX110815).
Western blot analysis was performed using ATP6V1A antibody (GTX110815).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

The data was published in the journal Front Immunol in 2019.PMID: 31781121