Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ATP6V1E1 antibody (GTX114056) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ATP6V1E1 antibody (GTX114056) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using ATP6V1E1(GTX114056) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

ATP6V1E1 antibody detects ATP6V1E1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
ATP6V1E1 stained by ATP6V1E1 antibody (GTX114056) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ATP6V1E1 antibody (GTX114056) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ATP6V1E1 antibody detects ATP6V1E1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ATP6V1E1 protein stained by ATP6V1E1 antibody (GTX114056) diluted at 1:200.
Blue: Hoechst 33342 staining.