Raji whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with ATR antibody (GTX128146) diluted at 1:500 and competitor's antibody (Highly cited Competitor ) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

ATR antibody detects ATR protein at nucleus on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
ATR antibody (GTX128146) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

ATR antibody detects ATR protein by western blot analysis. Raji whole cell extracts and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody (GTX128146) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ATR antibody detects ATR protein at nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
ATR antibody (GTX128146) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

ATR antibody detects ATR protein at nucleolus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATR protein stained by ATR antibody (GTX128146) diluted at 1:5000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Immunoprecipitation of ATR protein from HCT-116 whole cell extracts treated with Hydroxyurea for 6 hr using 5 μg of ATR antibody (GTX128146).
Western blot analysis was performed using ATR antibody (GTX128146).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

ATR antibody detects ATR protein by Western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg A431 whole cell extract
C. 30 μg HeLa whole cell extract
D. 30 μg HepG2 whole cell extract
5 % SDS-PAGE
ATR antibody (GTX128146) dilution: 1:500

The data was published in the journal Nucleic Acids Res in 2019. PMID: 31114877