Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with ATR (phospho Ser428) antibody [HL3246] (GTX640889) diluted at 1:1000 and ATR antibody (GTX70109) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Ser428) antibody [HL3246] (GTX640889) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ATR (phospho Ser428) antibody [HL3246] detects ATR (phospho Ser428) protein by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ATR (phospho Ser428) stained by ATR (phospho Ser428) antibody [HL3246] (GTX640889) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Untreated (–) and treated (+) mouse tissue extract (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Ser428) antibody [HL3246] (GTX640889) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.