Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody [HL132] (GTX635571) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

ATR (phospho Thr1989) antibody [HL132] detects ATR (phospho Thr1989) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody [HL132] (GTX635571) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

ATR (phospho Thr1989) antibody [HL132] detects ATR (phospho Thr1989) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody [HL132] (GTX635571) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

ATR (phospho Thr1989) antibody [HL132] detects ATR (phospho Thr1989) protein by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody [HL132] (GTX635571) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

ATR (phospho Thr1989) antibody [HL132] detects ATR (phospho Thr1989) protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissue.
ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody [HL132] (GTX635571) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

ATR (phospho Thr1989) antibody [HL132] detects ATR (phospho Thr1989) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat lung.
ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody [HL132] (GTX635571) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min