Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody [HL1836] (GTX637561) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ATR (phospho Thr1989) antibody [HL1836] detects ATR (phospho Thr1989) protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody [HL1836] (GTX637561) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).