Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATXN2 antibody [HL1902] (GTX637651) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ATXN2 antibody [HL1902] detects ATXN2 protein at cytoplasm by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATXN2 stained by ATXN2 antibody [HL1902] (GTX637651) diluted at 1:250.
Red: Tau, a axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

ATXN2 antibody [HL1902] detects ATXN2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
ATXN2 stained by ATXN2 antibody [HL1902] (GTX637651) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

ATXN2 antibody [HL1902] detects ATXN2 protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATXN2 stained by ATXN2 antibody [HL1902] (GTX637651) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).