Non-transfected (–) and transfected (+) transfected 293T (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATXN2 antibody [HL1903] (GTX637652) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ATXN2 antibody [HL1903] (GTX637652) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.