Non-transfected (–) and transfected (+) HeLa whole cell extracts (60 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AXL antibody [C2C3], C-term (GTX101345) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 ug of whole cell lysate)
A: H1299
5% SDS PAGE
GTX101345 diluted at 1:500

Western Blot analysis of AXL expression in transfected 293T cell line by AXL polyclonal antibody.
A: Non-transfected lysate.
B: AXL transfected lysate.
7.5% SDS PAGE
GTX101345 diluted at 1:500

Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using AXL(GTX101345) antibody at 1:200 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min