Non-transfected (–) and transfected (+) HeLa whole cell extracts (60 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AXL antibody (GTX129407) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

AXL antibody detects AXL protein at membrane on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
AXL antibody (GTX129407) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

AXL antibody detects AXL protein at cell membrane in human lung cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Green: AXL antibody (GTX129407) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Blue: Nuclear staining with Hoechst 33342.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with AXL antibody (GTX129407) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

An ELISA plate is coated with 50 μL of AXL recombinant protein at concentration of 4 μg/mL. The coated protein is detected with anti-AXL antibody (GTX129407) at concentration ranged from 0.25 to 1000 ng/mL.

AXL antibody detects AXL protein at cell membrane on human non-small cell lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human non-small cell lung carcinoma.
AXL antibody (GTX129407) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

AXL antibody detects AXL protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg whole cell extract of human AXL-transfected 293T cells
7.5 % SDS-PAGE
AXL antibody (GTX129407) dilution: 1:5000

AXL antibody detects AXL protein at membrane on human non-small cell lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human non-small cell lung carcinoma.
AXL antibody (GTX129407) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min