Non-transfected (–) and transfected (+) 293T whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Acylglycerol kinase antibody [HL1846] (GTX637571) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Acylglycerol kinase antibody [HL1846] (GTX637571) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.