WB analysis of whole cell extracts (30 μg lysate) of PC 12 (Lane 1), SH-SY5Y (lane 2), U-87MG (lane 3), A549 (Lane 4) and K-562 (lane 5) using GTX23460 Adenosine A1 Receptor antibody.
Dilution : 1:250-1:1000

ICC/IF analysis of HeLa cells using GTX23460 Adenosine A1 Receptor antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature

IHC-P analysis of rat brain tissue using GTX23460 Adenosine A1 Receptor antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:100