IHC-P analysis of human colon carcinoma tissue using GTX80683 Adiponectin antibody [19F1].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

WB analysis of 10 μl of conditioned media of HepG2 cell using GTX80683 Adiponectin antibody [19F1].
Dilution : 2 μg/ml

IHC-P analysis of human skin tissue using GTX80683 Adiponectin antibody [19F1].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

WB analysis of rabbit subcutaneous white adipose tissue (scWAT), gonadal white adipose tissue (gWAT), perirenal white adipose tissue (pWAT), tibia marrow adipose tissue (tibMAT), tibia red marrow (tibRM), and 3T3-L1 positive control adipocyte lysates using GTX80683 Adiponectin antibody [19F1].
Dilution : 1:1000

ICC/IF analysis of differentiated 3T3-L1 cells (treated with StemPro Adipogenesis Supplement for 5 days) using GTX80683 Adiponectin antibody [19F1]. Panel e is untreated cell with less signal. Panel f represents control cells with no primary antibody to assess background.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA incubated for 3 hours at room temperature