APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
Raji , Neuro2A , NIH-3T3 , Raw264.7 , C2C12 , mouse white adipose , PC-12 , Rat-2
Predict Reactivity
Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.62 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Alcohol dehydrogenase 1B. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2036175
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
alcohol dehydrogenase 1B (class I), beta polypeptide , ADH2 , HEL-S-117
Cellular Localization
Cytoplasm
Background
The protein encoded by this gene is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This encoded protein, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-Alcohol dehydrogenase 1B antibody [N2C3] used in Western Blot (WB). GTX114054](/templates/default/js/lightbox/images/loading.gif "Anti-Alcohol dehydrogenase 1B antibody [N2C3] used in Western Blot (WB). GTX114054")
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GTX114054 WB Image
Drosophila tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Alcohol dehydrogenase 1B antibody [N2C3] (GTX114054) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX114054 WB Image
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Alcohol dehydrogenase 1B antibody [N2C3] (GTX114054) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX114054 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Alcohol dehydrogenase 1B antibody [N2C3] (GTX114054) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX114054 WB Image
Sample (30 ug of whole cell lysate)
A: Raji
10% SDS PAGE
GTX114054 diluted at 1:1000
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GTX114054 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Alcohol dehydrogenase 1B antibody [N2C3] (GTX114054) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for Alcohol dehydrogenase 1B antibody [N2C3] (GTX114054). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Alcohol dehydrogenase 1B antibody [N2C3] (GTX114054). Be the first to share your experience with this product.
![Anti-Alcohol dehydrogenase 1B antibody [N2C3] used in Western Blot (WB). GTX114054](/upload/website/prouct_img/small/GTX114054/GTX114054_40150_20230324_WB_Drosophila_brain_23032819_886.webp "Anti-Alcohol dehydrogenase 1B antibody [N2C3] used in Western Blot (WB). GTX114054")
![Anti-Alcohol dehydrogenase 1B antibody [N2C3] used in Western Blot (WB). GTX114054](/upload/website/prouct_img/small/GTX114054/GTX114054_40150_20181221_WB_M_white_adipose_w_23060501_794.webp "Anti-Alcohol dehydrogenase 1B antibody [N2C3] used in Western Blot (WB). GTX114054")
![Anti-Alcohol dehydrogenase 1B antibody [N2C3] used in Western Blot (WB). GTX114054](/upload/website/prouct_img/small/GTX114054/GTX114054_40150_20181221_WB_R_w_23060501_469.webp "Anti-Alcohol dehydrogenase 1B antibody [N2C3] used in Western Blot (WB). GTX114054")
![Anti-Alcohol dehydrogenase 1B antibody [N2C3] used in Western Blot (WB). GTX114054](/upload/website/prouct_img/small/GTX114054/GTX114054_WB_w_23060501_518.webp "Anti-Alcohol dehydrogenase 1B antibody [N2C3] used in Western Blot (WB). GTX114054")
![Anti-Alcohol dehydrogenase 1B antibody [N2C3] used in Western Blot (WB). GTX114054](/upload/website/prouct_img/small/GTX114054/GTX114054_40150_20181221_WB_M_w_23060501_637.webp "Anti-Alcohol dehydrogenase 1B antibody [N2C3] used in Western Blot (WB). GTX114054")
![Anti-Alcohol dehydrogenase 1B antibody [N2C3] used in Western Blot (WB). GTX114054](/upload/website/prouct_img/normal/GTX114054/GTX114054_40150_20230324_WB_Drosophila_brain_23032819_886.webp "Anti-Alcohol dehydrogenase 1B antibody [N2C3] used in Western Blot (WB). GTX114054")
![Anti-Alcohol dehydrogenase 1B antibody [N2C3] used in Western Blot (WB). GTX114054](/upload/website/prouct_img/normal/GTX114054/GTX114054_40150_20181221_WB_M_white_adipose_w_23060501_794.webp "Anti-Alcohol dehydrogenase 1B antibody [N2C3] used in Western Blot (WB). GTX114054")
![Anti-Alcohol dehydrogenase 1B antibody [N2C3] used in Western Blot (WB). GTX114054](/upload/website/prouct_img/normal/GTX114054/GTX114054_40150_20181221_WB_R_w_23060501_469.webp "Anti-Alcohol dehydrogenase 1B antibody [N2C3] used in Western Blot (WB). GTX114054")
![Anti-Alcohol dehydrogenase 1B antibody [N2C3] used in Western Blot (WB). GTX114054](/upload/website/prouct_img/normal/GTX114054/GTX114054_WB_w_23060501_518.webp "Anti-Alcohol dehydrogenase 1B antibody [N2C3] used in Western Blot (WB). GTX114054")
![Anti-Alcohol dehydrogenase 1B antibody [N2C3] used in Western Blot (WB). GTX114054](/upload/website/prouct_img/normal/GTX114054/GTX114054_40150_20181221_WB_M_w_23060501_637.webp "Anti-Alcohol dehydrogenase 1B antibody [N2C3] used in Western Blot (WB). GTX114054")