IP analysis of various samples using GTX26190 Aldolase A antibody.
Lane 1 : Purified aldolase
Lane 2 : Serum without aldolase
Lane 3 : Serum with 100 μL aldolase
Lane 4 : Serum with 200 μL aldolase

WB analysis of various samples using GTX26190 Aldolase A antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Loading : 25 μg
Dilution : 1:1000

WB analysis of A293 whole cell lysate using GTX26190 Aldolase A antibody.
Dilution : 1:1000

IP analysis of various samples using GTX26190 Aldolase A antibody.
Lane 1 : Purified aldolase
Lane 2 : Serum without aldolase
Lane 3 : Serum with 20 μL aldolase
Lane 4 : Serum with 80 μL aldolasee
Lane 5 : Serum with 120 μL aldolasee
Lane 6 : Serum with 150 μL aldolasee
Lane 7 : Serum with 300 μL aldolase

IgG purified antibody to rabbit muscle aldolase (GTX26190) was used at a 1:1000 dilution to detect human aldolase by Western blot. A whole cell lysate prepared from human derived A293 cells was loaded on a 4-12% tris glycine gradient gel for SDS-PAGE. The gel was transferred to nitro-cellulose using standard techniques. Antibody reaction with the membrane occurred overnight at 4º C in TTBS supplemented with 2% non-fat dry milk. Color was allowed to develop using SuperSignal West Pico Chemiluminescent Substrate (PIERCE). Other detection methods will yield similar results. This antibody clearly detects a band at ~41 kDa consistent with human aldolase.