Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Aldolase B antibody (GTX114914) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: JurKat
D: Raji
10% SDS PAGE
GTX114914 diluted at 1:1000

Aldolase B antibody detects ALDOB protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma tissue.
Aldolase B antibody (GTX114914) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of methanol-fixed A431, using Aldolase B(GTX114914) antibody at 1:200 dilution.

Sample (20 ug of whole cell lysate)
A: mouse liver
10% SDS PAGE
GTX114914 diluted at 1:10000

Aldolase B antibody detects ALDOB protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 ug Rat2 whole cell lysate/extract
10 % SDS-PAGE
Aldolase B antibody (GTX114914) dilution: 1:1000

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Aldolase B antibody (GTX114914) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Aldolase B antibody (GTX114914) at 1:300 dilution.

Sample (30 μg of whole cell lysate)
A: zebrafish eye
10% SDS PAGE
GTX114914 diluted at 1:1000