APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Product Note
This antibody is specific for human ALPL protein, and it does not cross react with human ALPI, ALPP, and ALPG protein.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of Alkaline Phosphatase (Tissue Non-Specific).
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
alkaline phosphatase, biomineralization associated , AP-TNAP , APTNAP , HOPS , TNALP , TNAP , TNSALP
Cellular Localization
Cell membrane
Background
This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects. [provided by RefSeq, Oct 2015]
Database
Research Area
DATA IMAGES
![Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] used in Western Blot (WB). GTX637904](/templates/default/js/lightbox/images/loading.gif "Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] used in Western Blot (WB). GTX637904")
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GTX637904 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (GTX637904) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637904 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (GTX637904) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX637904 WB Image
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (GTX637904) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637904 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (GTX637904) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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REFERENCE
There are currently no references for Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (GTX637904). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (GTX637904). Be the first to share your experience with this product.
![Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] used in Western Blot (WB). GTX637904](/upload/website/prouct_img/small/GTX637904/GTX637904_T-44872_20230303_WB_multiple_B_23030717_526.webp "Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] used in Western Blot (WB). GTX637904")
![Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] used in Western Blot (WB). GTX637904](/upload/website/prouct_img/small/GTX637904/GTX637904_45012_20230804_WB_23080901_450.webp "Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] used in Western Blot (WB). GTX637904")
![Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] used in Western Blot (WB). GTX637904](/upload/website/prouct_img/small/GTX637904/GTX637904_45012_20230505_WB_23050918_741.webp "Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] used in Western Blot (WB). GTX637904")
![Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] used in Western Blot (WB). GTX637904](/upload/website/prouct_img/small/GTX637904/GTX637904_45012_20230616_WB_D_23062019_536.webp "Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] used in Western Blot (WB). GTX637904")
![Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] used in Western Blot (WB). GTX637904](/upload/website/prouct_img/normal/GTX637904/GTX637904_T-44872_20230303_WB_multiple_B_23030717_526.webp "Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] used in Western Blot (WB). GTX637904")
![Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] used in Western Blot (WB). GTX637904](/upload/website/prouct_img/normal/GTX637904/GTX637904_45012_20230804_WB_23080901_450.webp "Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] used in Western Blot (WB). GTX637904")
![Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] used in Western Blot (WB). GTX637904](/upload/website/prouct_img/normal/GTX637904/GTX637904_45012_20230505_WB_23050918_741.webp "Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] used in Western Blot (WB). GTX637904")
![Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] used in Western Blot (WB). GTX637904](/upload/website/prouct_img/normal/GTX637904/GTX637904_45012_20230616_WB_D_23062019_536.webp "Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] used in Western Blot (WB). GTX637904")