Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody (GTX100817) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft , using alkaline phosphatase (liver/bone/kidney)(GTX100817) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody (GTX100817) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.