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Alkaline phosphatase antibody [HL1267]

Recombinant antibody Overexpression
Anti-Alkaline phosphatase antibody [HL1267] used in Western Blot (WB). GTX636669
Anti-Alkaline phosphatase antibody [HL1267] used in IHC (Paraffin sections) (IHC-P). GTX636669
Anti-Alkaline phosphatase antibody [HL1267] used in Western Blot (WB). GTX636669
Anti-Alkaline phosphatase antibody [HL1267] used in Western Blot (WB). GTX636669

Cat. No. GTX636669

Reference 1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1267

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Alkaline phosphatase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Alkaline phosphatase. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910037

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

alkaline phosphatase, intestinal , IAP

Background

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is a component of the gut mucosal defense system and is thought to function in the detoxification of lipopolysaccharide, and in the prevention of bacterial translocation in the gut. [provided by RefSeq, Dec 2014]

Database

Research Area

DATA IMAGES

Anti-Alkaline phosphatase antibody [HL1267] used in Western Blot (WB). GTX636669

GTX636669 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline phosphatase antibody [HL1267] (GTX636669) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Alkaline phosphatase antibody [HL1267] used in IHC (Paraffin sections) (IHC-P). GTX636669

GTX636669 IHC-P Image

Alkaline phosphatase antibody [HL1267] detects Alkaline phosphatase protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Alkaline phosphatase stained by Alkaline phosphatase antibody [HL1267] (GTX636669) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Alkaline phosphatase antibody [HL1267] used in Western Blot (WB). GTX636669

GTX636669 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline phosphatase antibody [HL1267] (GTX636669) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Alkaline phosphatase antibody [HL1267] used in Western Blot (WB). GTX636669

GTX636669 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Alkaline phosphatase (intestinal) antibody [HL1267] (GTX636669) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Steiner D et al. Cells 2022; 11 (6) Impact of Endothelial Progenitor Cells in the Vascularization of Osteogenic Scaffolds.
Application : IHC-P
Reactivity : Mouse
Submit a Reference

REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations