APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human Angiogenin.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
angiogenin , ALS9 , HEL168 , RAA1 , RNASE4 , RNASE5
Cellular Localization
Secreted , Nucleus , Nucleolus
Background
The protein encoded by this gene is an exceedingly potent mediator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. In addition, the mature peptide has antimicrobial activity against some bacteria and fungi, including S. pneumoniae and C. albicans. Alternative splicing results in two transcript variants encoding the same protein. This gene and the gene that encodes ribonuclease, RNase A family, 4 share promoters and 5' exons. Each gene splices to a unique downstream exon that contains its complete coding region. [provided by RefSeq, Aug 2014]
Database
Research Area
DATA IMAGES
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GTX638218 WB Image
HepG2 whole cell extract and conditioned medium (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Angiogenin antibody [HL2214] (GTX638218) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638218 IHC-P Image
Angiogenin antibody [HL2214] detects Angiogenin protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.Angiogenin stained by Angiogenin antibody [HL2214] (GTX638218) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638218 ICC/IF Image
Angiogenin antibody [HL2214] detects Angiogenin protein at cytoplasm by immunofluorescent analysis.Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Angiogenin stained by Angiogenin antibody [HL2214] (GTX638218) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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REFERENCE
There are currently no references for Angiogenin antibody [HL2214] (GTX638218). Be the first to share your publications with this product.
REVIEW
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