Wild-type (WT) and Annexin A1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody (GTX113329) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Annexin A1 antibody (GTX113329) diluted at 1:5000 and competitor's antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Sample (30 ug of whole cell lysate)
A: H1299
10% SDS PAGE
GTX113329 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Annexin A1(GTX113329) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Annexin A1(GTX113329) antibody at 1:200 dilution.

Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX113329 diluted at 1:10000