Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Anterior Gradient 2 antibody [HL1024] (GTX635838) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Anterior Gradient 2 antibody [HL1024] (GTX635838) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anterior Gradient 2 antibody [HL1024] detects Anterior Gradient 2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissue.
Anterior Gradient 2 stained by Anterior Gradient 2 antibody [HL1024] (GTX635838) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anterior Gradient 2 antibody [HL1024] detects Anterior Gradient 2 protein at cytoplasm by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: Anterior Gradient 2 stained by Anterior Gradient 2 antibody [HL1024] (GTX635838) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).