Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Anterior Gradient 2 antibody (GTX130229) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunoprecipitation of AGR2 protein from MCF-7 whole cell extracts using 5 μg of AGR2 antibody (GTX130229).
Western blot analysis was performed using AGR2 antibody (GTX130229).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

AGR2 antibody detects AGR2 protein at cytoplasm in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
AGR2 antibody (GTX130229) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

AGR2 antibody detects AGR2 protein at cytoplasm by immunofluorescent analysis.
Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: AGR2 protein stained by AGR2 antibody (GTX130229) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.