WB analysis of HeLa whole cell lysate using GTX23629 Apc10 antibody.
Lane 1 : whole cell lysate
Lane 2 : Nuclear extract
Loading : 20 μg
Dilution : 1:500

WB analysis of various samples using GTX23629 Apc10 antibody.
Lane 1 : HeLa cell nuclear extract
Lane 2 : HeLa whole cell lysate
Lane 3 : A431 whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lane 5 : 293T whole cell lysate
Loading : 20 μg
Dilution : 1:500

Affinity Purified Rabbit anti-APC10 (GTX23629) was used at a 1:500 dilution to detect human APC10 in various cell extracts. This antibody clearly detects a ~26 kDa band corresponding to human APC10 (predicted molecular weight is 21 kDa). All lanes contain 20 ug of lysate or extract as follows: lane 1, HeLa nuclear extract; lane 2, HeLa whole cell lysate; lane 3, A431 whole cell lysate; lane 4, Jurkat whole cell lysate; lane 5, 293 whole cell lysate. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 4 min.

Western blotting. Affinity Purified Rabbit anti-APC10 (GTX23629) was used at a 1:500 dilution to detect human APC10 by western blot. Both HeLa whole cell lysate (lane 1) and nuclear lysate (lane 2) were probed using this antibody. Approximately 20 ug of each lysate was loaded onto a 10% SDS-PAGE gel. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:2,000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 5 min. The expected molecular weight of human APC10 is 21 kDa