Mouse plasma and Rat plasma (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Apolipoprotein A1 antibody (GTX112692) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Human plasma (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Apolipoprotein A1 antibody (GTX112692) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Apolipoprotein A1 antibody detects secreted Apolipoprotein A1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human esophagus.
Apolipoprotein A1 stained by Apolipoprotein A1 antibody (GTX112692) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Apolipoprotein A1 antibody detects secreted Apolipoprotein A1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
Apolipoprotein A1 stained by Apolipoprotein A1 antibody (GTX112692) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Apolipoprotein A1 antibody detects secreted Apolipoprotein A1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human lung.
Apolipoprotein A1 stained by Apolipoprotein A1 antibody (GTX112692) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Apolipoprotein A1 antibody detects secreted Apolipoprotein A1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human colon.
Apolipoprotein A1 stained by Apolipoprotein A1 antibody (GTX112692) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Apolipoprotein A1 antibody detects Apolipoprotein A1 protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Apolipoprotein A1 stained by Apolipoprotein A1 antibody (GTX112692) diluted at 1:500.
Blue: Hoechst 33342 staining.

Apolipoprotein A1 antibody detects Apolipoprotein A1 protein at Golgi apparatus by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Apolipoprotein A1 stained by Apolipoprotein A1 antibody (GTX112692) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

The data was published in the 2022 in Biomedicines. PMID: 35203448