Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:2000 |
1:100 |
1:4000-1:8000 |
1:50-1:200 |
Not tested in other applications.
Calculated MW
Product Note
Typically less than 1% cross-reactivity against other types of apolipoprotein was detected by ELISA against purified standards. This antibody reacts with human apolipoprotein E and has negligible cross-reactivity with Type A-1 A-II, B, C-I, C-II, C-III and J apolipoproteins. Specific cross-reaction of anti-apolipoprotein antibodies with antigens from other species has not been determined. Non-specific cross-reaction of anti-apolipoprotein antibodies with other human serum proteins is negligible.
Form
Liquid
Buffer
125mM Sodium Borate, 75mM NaCl, 5mM EDTA
Preservative
0.01% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Apolipoprotein Type E was isolated from human plasma by density gradient centrifugation followed by HPLC purification.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
apolipoprotein E , AD2 , APO-E , ApoE4 , LDLCQ5 , LPG
Cellular Localization
Secreted
Background
At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). The apolipoproteins are derived from human plasma by density gradient ultracentrifugation and HPLC.
Database
Research Area