Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Aquaporin 4 antibody (GTX133151) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Aquaporin 4 antibody detects Aquaporin 4 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: Aquaporin 4 protein stained by Aquaporin 4 antibody (GTX133151) diluted at 1:250.
Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Aquaporin 4 antibody detects Aquaporin 4 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Aquaporin 4 stained by Aquaporin 4 antibody (GTX133151) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Aquaporin 4 antibody detects Aquaporin 4 protein at astrocyte cells by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Aquaporin 4 protein stained by Aquaporin 4 antibody (GTX133151) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Aquaporin 4 antibody detects Aquaporin 4 protein at cell membrane in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Aquaporin 4 antibody (GTX133151) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min