Wild-type (WT) and ATXN3 knockout (KO) 293T cell extracts (30 μg) were separated by gradient gel% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody (GTX101343) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.<br><I>Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.</I>

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody (GTX101343) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Ataxin 3 antibody detects Ataxin 3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10 % SDS-PAGE, and blotted with Ataxin 3 antibody (GTX101343) diluted by 1:1000

Ataxin 3 antibody detects Ataxin 3 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Ataxin 3 protein stained by Ataxin 3 antibody (GTX101343) diluted at 1:250.
Blue: Hoechst 33342 staining.

Ataxin 3 antibody detects Ataxin 3 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Ataxin 3 protein stained by Ataxin 3 antibody (GTX101343) diluted at 1:250.
Blue: Hoechst 33342 staining.

Ataxin 3 antibody detects Ataxin 3 protein by western blot analysis.
293T mock transfected (-) or transfected with a construct expressing human Ataxin 3.
10 % SDS-PAGE
Ataxin 3 antibody (GTX101343) dilution: 1:20000