APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human Aurora A.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
aurora kinase A , AIK , ARK1 , AURA , BTAK , PPP1R47 , STK15 , STK6 , STK7
Cellular Localization
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Background
The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-Aurora A antibody [HL2917] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX640260](/templates/default/js/lightbox/images/loading.gif "Anti-Aurora A antibody [HL2917] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX640260")
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GTX640260 ICC/IF Image
Aurora A antibody [HL2917] detects Aurora A protein by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Aurora A stained by Aurora A antibody [HL2917] (GTX640260) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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GTX640260 WB Image
Untreated (–) and treated (+) HeLa whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Aurora A antibody [HL2917] (GTX640260) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for Aurora A antibody [HL2917] (GTX640260). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Aurora A antibody [HL2917] (GTX640260). Be the first to share your experience with this product.
![Anti-Aurora A antibody [HL2917] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX640260](/upload/website/prouct_img/small/GTX640260/GTX640260_T-45390_20240510_ICC_IF_24052202_353.webp "Anti-Aurora A antibody [HL2917] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX640260")
![Anti-Aurora A antibody [HL2917] used in Western Blot (WB). GTX640260](/upload/website/prouct_img/small/GTX640260/GTX640260_45460_20240705_WB_treatment_Nocadazole_24070822_575.webp "Anti-Aurora A antibody [HL2917] used in Western Blot (WB). GTX640260")
![Anti-Aurora A antibody [HL2917] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX640260](/upload/website/prouct_img/normal/GTX640260/GTX640260_T-45390_20240510_ICC_IF_24052202_353.webp "Anti-Aurora A antibody [HL2917] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX640260")
![Anti-Aurora A antibody [HL2917] used in Western Blot (WB). GTX640260](/upload/website/prouct_img/normal/GTX640260/GTX640260_45460_20240705_WB_treatment_Nocadazole_24070822_575.webp "Anti-Aurora A antibody [HL2917] used in Western Blot (WB). GTX640260")