Indirect ELISA analysis was performed by coating the plate with recombinant HEK293 expressed, full-length Avian Influenza A virus HSN8 HA (Hemagglutinin) protein (63.49-0.99 nM). Coated protein was probed with Avian Influenza A virus H5N8 HA (Hemagglutinin) antibody [HL1550] (GTX637025) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Avian Influenza A virus H5N8 HA (Hemagglutinin) antibody [HL1550] (GTX637025) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected and transfected 293T whole cell extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with Avian Influenza A virus H5N8 HA (Hemagglutinin) antibody [HL1550] (GTX637025) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Avian Influenza A virus H5N8 HA (Hemagglutinin) antibody [HL1550] detects Avian Influenza A virus H5N8 HA (Hemagglutinin) protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mock and Avian Influenza A virus H5N8 HA - transfected 293T cell pellet. Green: Avian Influenza A virus H5N8 HA (Hemagglutinin) stained by Avian Influenza A virus H5N8 HA (Hemagglutinin) antibody [HL1550] (GTX637025) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min