Wild-type (WT) and BRAF knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B-Raf antibody [HL1606] (GTX637085) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

B-Raf antibody [HL1606] detects B-Raf protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
B-Raf stained by B-Raf antibody [HL1606] (GTX637085) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

B-Raf antibody [HL1606] detects B-Raf protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
B-Raf stained by B-Raf antibody [HL1606] (GTX637085) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B-Raf antibody [HL1606] (GTX637085) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

B-Raf antibody [HL1606] detects B-Raf protein at cytoplasm by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: B-Raf stained by B-Raf antibody [HL1606] (GTX637085) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

B-Raf antibody [HL1606] detects B-Raf protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
B-Raf stained by B-Raf antibody [HL1606] (GTX637085) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B-Raf antibody [HL1606] (GTX637085) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

B-Raf antibody [HL1606] detects B-Raf protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
B-Raf stained by B-Raf antibody [HL1606] (GTX637085) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B-Raf antibody [HL1606] (GTX637085) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.