Wild-type (WT) and BRAF knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B-Raf antibody [HL1735] (GTX637383) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B-Raf antibody [HL1735] (GTX637383) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

B-Raf antibody [HL1735] detects B-Raf protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
B-Raf stained by B-Raf antibody [HL1735] (GTX637383) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min