B-Raf antibody [N2C1], Internal detects B-Raf protein by western blot analysis.
A. 30 μg PC-12 whole cell extract
7.5 % SDS-PAGE
B-Raf antibody [N2C1], Internal (GTX100913) dilution: 1:5000

B-Raf antibody [N2C1], Internal detects B-Raf protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
B-Raf stained by B-Raf antibody [N2C1], Internal (GTX100913) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

B-Raf antibody [N2C1], Internal detects B-Raf protein by western blot analysis. Non-transfected (-) and B-Raf-transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B-Raf antibody [N2C1], Internal (GTX100913) diluted at 1:5000.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B-Raf antibody [N2C1], Internal (GTX100913) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

B-Raf antibody [N2C1], Internal immunoprecipitates B-Raf protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 μg whole cell lysate/extract of B-Raf protein expressing HepG2 cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of B-Raf by 3 μg of B-Raf antibody [N2C1], Internal (GTX100913) 10% SDS-PAGE The immunoprecipitated B-Raf protein was detected by B-Raf antibody [N2C1], Internal (GTX100913) diluted at 1:500. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

B-Raf antibody [N2C1], Internal detects B-Raf protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
B-Raf antibody [N2C1], Internal (GTX100913) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min