Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BAF57 antibody [C3], C-term (GTX106345) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human lung, using BAF57(GTX106345) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample(30 μg of whole cell lysate)
A:Raji(GTX27908)
7.5% SDS PAGE
GTX106345 diluted at 1:3000

BAF57 antibody [C3], C-term detects BAF57 protein at nucleus by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BAF57 protein stained by BAF57 antibody [C3], C-term (GTX106345) diluted at 1:500.
Blue: Hoechst 33342 staining.