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BAFF antibody [Buffy-1]

Anti-BAFF antibody [Buffy-1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX16107
Anti-BAFF antibody [Buffy-1] used in Flow cytometry (FACS). GTX16107

Cat. No. GTX16107

Host

 Rat

Clonality

 Monoclonal

Clone Name

 Buffy-1

Isotype

 IgG2a

Application

 WB, ICC/IF, FACS

Reactivity

 Human
Package
50 μg ($399)
See all BAFF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
FACS Assay dependent

Note :

FACS
HEK 293T cells (5 x 10⁵) were mock transfected or transfected with BAFF.
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Product Note

Recognizes membrane-bound human BAFF. Detects endogenous protein by Flow Cytometry.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

BAFF (monoclonal Buffy-1) antibody was raised against recombinant human soluble BAFF (aa. 83-285).

Purification

Purified IgG2a

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TNF superfamily member 13b , BAFF , BLYS , CD257 , DTL , TALL-1 , TALL1 , THANK , TNFSF20 , TNLG7A , ZTNF4

Cellular Localization

Cell membrane; Single-pass type II membrane protein

Background

Members in the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated BAFF (for B cell Activating Factor belonging to the TNF Family), BLyS (for B Lymphocyte Stimulator), TALL1 (for TNF- and ApoL- related Leukocyte-expressed Ligand), and THANK (for TNF Homologue that Activate Apoptosis, NFkB and c-jun N-terminal Kinase). BAFF/BLyS was characterized as a B cell stimulator since it induced B cell proliferation and immunoglobulin secretion. Two receptors for BAFF were recently identified and designated TACI and BCMA. BAFF and its receptors are involved in the development of systemic lupus erythaematosus and other B cell associated autoimmune diseases. Like TNFa and TRAIL,THANK was shown to activate NF-kB and c-jun N terminal kinase (JNK) and to induce apoptosis.

Database

Research Area

DATA IMAGES

Anti-BAFF antibody [Buffy-1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX16107

GTX16107 ICC/IF Image

BAFF antibody [Buffy-1]

Anti-BAFF antibody [Buffy-1] used in Flow cytometry (FACS). GTX16107

GTX16107 FACS Image

It shows the flow cytometric profile of 293 cells stably transfected with a BAFF-expression plasmid (1). Control (2) are mock transfected 293 cells. Optimal concentration range is 0.5-5 μg/ml.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399