Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with BCA1 antibody [N3C3] (GTX108471) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

BCA1 antibody [N3C3] detects BCA1 protein at cytoplasm in human esophageal carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal carcinoma.
BCA1 antibody [N3C3] (GTX108471) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

BCA1 antibody [N3C3] detects BCA1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal carcinoma.
BCA1 stained by BCA1 antibody [N3C3] (GTX108471) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

BCA1 antibody [N3C3] detects secreted BCA1 protein by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BCA1 protein stained by BCA1 antibody [N3C3] (GTX108471) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

BCA1 antibody [N3C3] detects BCA1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal carcinoma.
BCA1 stained by BCA1 antibody [N3C3] (GTX108471) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

BCA1 antibody [N3C3] detects BCA1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal carcinoma.
BCA1 stained by BCA1 antibody [N3C3] (GTX108471) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

BCA1 antibody [N3C3] detects BCA1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal carcinoma.
BCA1 stained by BCA1 antibody [N3C3] (GTX108471) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min